Mouse Primary Aortic Endothelial Cells

This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment.

Mouse primary aortic endothelial cells.

Enos knockout mice were kindly provided by dr. C57bl 6 mouse primary aortic endothelial cells can be used in assays of cell cell adhesion migration vascular tube formation or transendothelial resistance ter. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology. Euthanatize mice by co 2 asphyxiation followed by cervical dislocation.

Isolation of mouse primary aortic endothelial cells by selection with specific antibodies methods mol biol. They produce endothelium derived substances regulating vasoconstriction and vessel growth. Cut off tissue to open chest and abdomen and expose aorta. Paul huang cardiovascular.

Marek drab max planck institute for molecular cell biology and genetics dresden germany. Wild type c57bl 6 and p47 phox knockout mice were purchased from jackson laboratories bar harbor maine. Endothelial cells ecs are key blood vessel wall components that play critical roles in the regulation of many physiological processes including angiogenesis coagulation and vascular tone. Primary human aortic endothelial cells or haoecs acbri 375 were initiated from normal human descending aorta aortic arch to renal artery.

Mouse aortic endothelial cells maec were isolated from the thoracic and abdominal aortas of various control and knockout mouse lines. In the study of vascular biology analyses of endothelial cells ec and smooth muscle cells smc are very important. Isolation of mouse primary aortic endothelial cells by selection with specific antibodies springerlink. Authors pedro molina sánchez 1 vicente andrés 2 affiliations 1 laboratory of molecular and genetic.

Method of primary cell culture with appropriate reagents. The mouse is a critical model for research however the isolation of primary ec from murine aorta is considered difficult. Caveolin 1 cav1 knockout mice were kindly provided by dr. The vascular endothelial cells play a significant role in many important cardiovascular disorders.

Mouse aortic endothelial cells were isolated from c57bl 6 mice and used for experiments without any passage. Wipe off skin with 70 isopropanol and fix mice on an operation table. Primary human aortic endothelial cells acbri 375 were initiated from normal human descending aorta aortic arch to renal artery. Previously reported procedures for the isolation of ec have required magnetic beads or fluorescence activated cell sorting facs to purify the cells.

The human umbilical vein endothelial cells were cultured by using cell passageway to further growth in the culture medium.